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Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar-to-Ductal Metaplasia

  • Jun Liu
  • Pei WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1882)

Abstract

Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.

Key words

Human exocrine pancreatic cells Acinar-to-ductal metaplasia Lineage tracing Flow cytometry 3D culture 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Cell Systems and AnatomyThe University of Texas Health San AntonioSan AntonioUSA

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