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Autophagy pp 679-690 | Cite as

Measuring Antibacterial Autophagy

  • Keith B. Boyle
  • Felix RandowEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Abstract

Bacteria that escape from membrane-enclosed vacuoles to the cytosol of cells are targeted by autophagy, which recognizes and captures bacteria into autophagosomes wherein their proliferation is restricted. Here we discuss two means by which antibacterial autophagy is assessed: (1) the visualization and enumeration of autophagy protein recruitment to the vicinity of cytosolic bacteria by means of immunofluorescence microscopy and (2) the measurement of autophagy-dependent restriction of bacterial proliferation by means of colony-forming unit assay.

Key words

Salmonella Galectin Ubiquitin Colony-forming unit Immunofluorescence 

Notes

Acknowledgments

This work was supported by the MRC (U105170648) and the Wellcome Trust (WT104752MA).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Protein and Nucleic Acid ChemistryMRC Laboratory of Molecular BiologyCambridgeUK
  2. 2.Department of MedicineAddenbrooke’s Hospital, University of CambridgeCambridgeUK

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