Autophagy pp 601-610 | Cite as

Imaging the Dynamics of Mitophagy in Live Cells

Part of the Methods in Molecular Biology book series (MIMB, volume 1880)


Investigating the precise spatiotemporal dynamics of mitophagy can provide insights into how mitochondrial quality control is regulated in different tissues and organisms. Here, we outline live imaging assays to quantitatively assess mitophagy dynamics in real time. This protocol describes both chemical and optogenetic techniques to induce mitochondrial damage with high spatial and temporal control. Using these assays, mitochondria can be tracked from before they sustain damage up to their engulfment by autophagosomes and acidification by lysosomes.

Key words

Mitophagy Parkin LC3 OPTN Autophagy Mitochondria Ubiquitin Lysosome 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of PhysiologyUniversity of Pennsylvania Perelman School of MedicinePhiladelphiaUSA

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