Imaging the Dynamics of Mitophagy in Live Cells
Investigating the precise spatiotemporal dynamics of mitophagy can provide insights into how mitochondrial quality control is regulated in different tissues and organisms. Here, we outline live imaging assays to quantitatively assess mitophagy dynamics in real time. This protocol describes both chemical and optogenetic techniques to induce mitochondrial damage with high spatial and temporal control. Using these assays, mitochondria can be tracked from before they sustain damage up to their engulfment by autophagosomes and acidification by lysosomes.
Key wordsMitophagy Parkin LC3 OPTN Autophagy Mitochondria Ubiquitin Lysosome
- 5.Wang Y, Nartiss Y, Steipe B, McQuibban AG, Kim PK (2012) ROS-induced mitochondrial depolarization initiates PARK2/PARKIN-dependent mitochondrial degradation by autophagy. Autophagy 8:1462–1476 http://www.tandfonline.com/doi/abs/10.4161/auto.21211CrossRefGoogle Scholar