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Autophagy pp 481-489 | Cite as

Analysis of Autophagy for Liver Pathogenesis

  • Nazmul Huda
  • Hui Zou
  • Shengmin Yan
  • Bilon Khambu
  • Xiao-Ming YinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)

Abstract

The autophagy pathway in hepatocytes is well characterized. Autophagy plays a critical role in the normal function of the liver. A growing number of studies suggest that there is a mechanistic relationship between autophagy and the pathogenesis of human diseases including liver diseases. Here we focus on the methods assessing the level of lipids, lipid peroxidation, and lipophagy in the liver, which would be particularly relevant to the study of fatty liver diseases.

Key words

Autophagy Fatty liver diseases Hepatocytes Immunofluorescence Lipid droplets Lipophagy Reactive oxygen species 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Nazmul Huda
    • 1
  • Hui Zou
    • 1
    • 2
  • Shengmin Yan
    • 1
  • Bilon Khambu
    • 1
  • Xiao-Ming Yin
    • 1
    Email author
  1. 1.Department of Pathology and Laboratory MedicineIndiana University School of MedicineIndianapolisUSA
  2. 2.College of Veterinary MedicineYangzhou UniversityYangzhouPeople’s Republic of China

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