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Automated Detection of Autophagy Response Using Single Cell-Based Microscopy Assays

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Autophagy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1880))

Abstract

The fluorescence microscopy-based detection of intracellular LC3, p62, and/or WIPI punctate structures is a robust tool to monitor and assess macroautophagy/autophagy in single cells. This method was established for automated high-throughput/content analysis to reliably detect narrow differences in autophagy activity/capacity and to provide screening opportunities for biological and chemical libraries.

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Acknowledgments

We apologize to researchers whose work we were unable to cite due to length constraints. Amelie J. Mueller received a predoctoral stipend from the International Max Planck Research School “From Molecules to Organisms.” Tassula Proikas-Cezanne receives grant support from the Deutsche Forschungsgemeinschaft (DFG), SFB/TRR 209 (project B02), and FOR 2625 (project 1).

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Authors and Affiliations

  1. Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen, Tübingen, Germany

    Amelie J. Mueller & Tassula Proikas-Cezanne

  2. International Max Planck Research School “From Molecules to Organisms”, Tübingen, Germany

    Amelie J. Mueller & Tassula Proikas-Cezanne

Authors
  1. Amelie J. Mueller
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  2. Tassula Proikas-Cezanne
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Correspondence to Tassula Proikas-Cezanne .

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Editors and Affiliations

  1. Signalling Programme, Babraham Institute, Cambridge, UK

    Nicholas Ktistakis

  2. Signalling Programme, Babraham Institute, Cambridge, UK

    Oliver Florey

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Mueller, A.J., Proikas-Cezanne, T. (2019). Automated Detection of Autophagy Response Using Single Cell-Based Microscopy Assays. In: Ktistakis, N., Florey, O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8873-0_27

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  • DOI: https://doi.org/10.1007/978-1-4939-8873-0_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8872-3

  • Online ISBN: 978-1-4939-8873-0

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