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Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP)

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Book cover BCL-2 Family Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1877))

Abstract

The BCL-2 family of proteins orchestrates a complex signaling network that governs the balance between cellular survival and death. A comprehensive understanding of the mechanistic interactions between these proteins continues to evolve in normal and malignant cells. The functional variation by individual BCL-2 proteins in different cell types has driven clinical therapeutic development in targeting individual BCL-2 members with the goal of fine-tuning cell death in diseased cells. Given the importance of understanding and validating the effect of activating or inhibiting BCL-2 protein interactions in individual cells, the methods used to measure apoptotic cell death have undergone increased scrutiny. Here, we describe two in vitro flow cytometry-based methods that are useful in measuring BCL-2 proteins and mitochondrial-based cell death in complex cell populations.

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Acknowledgment

We would like to thank Eric E. Smith for graphics in Fig. 2 and 5.

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Author notes

  1. Lindsey M. Ludwig and Katrina L. Maxcy contributed equally to the chapter.

Authors and Affiliations

  1. Section of Hematology/Oncology/Stem Cell Transplantation and Committee on Cancer Biology, Department of Pediatrics, University of Chicago, Chicago, IL, USA

    Lindsey M. Ludwig, Katrina L. Maxcy & James L. LaBelle

Authors
  1. Lindsey M. Ludwig
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  2. Katrina L. Maxcy
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  3. James L. LaBelle
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Corresponding author

Correspondence to James L. LaBelle .

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Editors and Affiliations

  1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA

    Evripidis Gavathiotis

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Ludwig, L.M., Maxcy, K.L., LaBelle, J.L. (2019). Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP). In: Gavathiotis, E. (eds) BCL-2 Family Proteins. Methods in Molecular Biology, vol 1877. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8861-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8861-7_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8860-0

  • Online ISBN: 978-1-4939-8861-7

  • eBook Packages: Springer Protocols

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