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Quantification of BCL-2 Family Members by Flow Cytometry

  • Morey L. Smith
  • Stephen K. Tahir
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1877)

Abstract

Flow cytometry is a powerful technique for the detection and quantification of cell surface and intracellular proteins. It enables the ability to measure the expression levels of specific proteins in a cell population of interest without the need to physically separate out the cells from within a heterogeneous population by using the appropriate cell-specific markers. It also requires fewer cells than other traditional techniques such as Western blotting. Here we describe a robust and reproducible method to measure the expression levels of the BCL-2 family members, BCL-2, BCL-XL, and MCL-1 by quantitative flow cytometry (QFCM) using validated antibodies.

Key words

Quantitative flow cytometry method Molecules of equivalent soluble fluorochrome BCL-2 MCL-1 BCL-XL 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Research and Development, Oncology DiscoveryAbbVie, Inc.North ChicagoUSA

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