Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene

  • Assunta BertacciniEmail author
  • Samanta Paltrinieri
  • Nicoletta Contaldo
Part of the Methods in Molecular Biology book series (MIMB, volume 1875)


Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.

Key words

Phytoplasma Detection Identification Ribosomal group Ribosomal subgroup Sequencing 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Assunta Bertaccini
    • 1
    Email author
  • Samanta Paltrinieri
    • 1
  • Nicoletta Contaldo
    • 1
  1. 1.Phytobacteriology Laboratory, DISTAL, Alma Mater StudiorumUniversity of BolognaBolognaItaly

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