Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation
The CRISPR-Cas9 system in bacteria and archaea has recently been exploited for genome editing in various model organisms, including the mice. In this scheme, components of the CRISPR-Cas9 system are delivered into the mouse zygote and mutant mice carrying genetic modifications derived. Although microinjection has been the technology of choice, electroporation has also emerged and been proven to be effective delivering CRISPR-Cas9 reagents into the mouse zygote. Here, we describe the experimental protocol employing electroporation to deliver CRISPR-Cas9 reagents into mouse embryos and derive gene-edited mutant mice.
Key wordsCRISPR-Cas9 Electroporation Genome editing Mouse models
We would like to thank the Genetic Engineering Technologies and the Reproductive sciences groups of the Jackson Laboratory for their partnership and contribution exploring into the CRISPR-Cas9 technology. Research reported in this publication was partially supported by the National Cancer Institute under award number P30CA034196. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. H.W. is supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA16010205), “National Natural Science Foundation of China” (31471215), and the National Key Research and Development Program of China (No. 2016YFA0101402).
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