Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System
Antibodies specifically recognizing integral membrane protein are essential tool for functional analysis, diagnosis, and therapeutics targeting membrane proteins. However, development of antibodies against membrane protein remains a big challenge, because mass production of membrane protein is difficult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using cell-free protein synthesis method with liposome and dialysis cup. Here we introduce practical and efficient integrated procedures to produce large amount of proteoliposome antigen for anti-membrane protein antibody development.
Key wordsProteoliposome Membrane protein Cell-free protein synthesis Immunizing antigen Adjuvant
The authors thank Mr. Tomio Ogasawara for his assistance in the technological development. We also thank Professor Tatsuya Sawasaki for his mentoring. This work was mainly supported by Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED, Japan. This work was also partially supported by JSPS KAKENHI Grant Numbers 24710251 and 26750375 and 16k01915.
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