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Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen

  • Hiroyuki TakedaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1868)

Abstract

Autoantibodies that recognize self-antigens are believed to have close relationship diseases such as autoimmune diseases, cancer, and lifestyle diseases. Analysis of autoantibodies is essential for investigating pathology mechanisms, diagnosis, and therapeutics of these diseases. We developed autoantibody profiling assay using cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitively detect interaction between recombinant antigen protein and autoantibody and efficiently analyze autoantibody profiling in patients’ sera.

Key words

Autoantibody Autoantigen Protein array AlphaScreen Autoantibody profiling Wheat cell-free protein synthesis 

Notes

Acknowledgments

The author would like to thank all collaborators and colleagues who conduct autoantibody profiling assay together. The author thanks Dr. Yohei Miyagi for his bioresource collection, Professor Tatsuya Sawasaki for his mentoring, and Wei Zhou for proofreading and valuable comments. This work was partially supported by Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED, Japan.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Proteo-Science CenterEhime UniversityMatsuyamaJapan

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