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Delivery of Superoxide Dismutase Using Cys2-His2 Zinc-Finger Proteins

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Zinc Finger Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1867))

Abstract

Therapeutic proteins have shown great potential in treating life-threatening diseases, but the hydrophilicity and high molecular weight hamper their passing through the cell membrane. Cell-penetrating peptide (CPP)-assisted protein delivery is a simple and efficacious strategy to promote the cellular uptake of therapeutic proteins. We recently demonstrated that the engineered Cys2-His2 zinc-finger domains possess intrinsic cell permeability, which could be leveraged for intracellular protein delivery. Here we applied this method to deliver superoxide dismutase (SOD), a therapeutic protein widely used in preclinical and clinical studies. We present a protocol for the production and delivery of zinc-finger domain-fused SOD. This protocol can be extended for delivering other therapeutic proteins.

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Acknowledgments

This work was supported by ShanghaiTech University, the National Natural Science Foundation of China (31500632 to P. Ma), and National Natural Science Foundation of China (31600686 to J. Liu).

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Authors and Affiliations

  1. Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai, China

    Jia Liu, Jiangmei Li, Jie Li, Lianhui Zhu, Shaojie Wang, Xuan Wei & Peixiang Ma

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  1. Jia Liu
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  2. Jiangmei Li
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  3. Jie Li
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  4. Lianhui Zhu
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  5. Shaojie Wang
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Correspondence to Peixiang Ma .

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  1. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China

    Jia Liu

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Liu, J. et al. (2018). Delivery of Superoxide Dismutase Using Cys2-His2 Zinc-Finger Proteins. In: Liu, J. (eds) Zinc Finger Proteins. Methods in Molecular Biology, vol 1867. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8799-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8799-3_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8798-6

  • Online ISBN: 978-1-4939-8799-3

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