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Pilot Phase I Clinical Trial of Methioninase on High-Stage Cancer Patients: Rapid Depletion of Circulating Methionine

  • Robert M. HoffmanEmail author
  • Yuying Tan
  • Shukuan Li
  • Qinghong Han
  • Jorge ZavalaSr
  • Jorge ZavalaJr
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1866)

Abstract

Methionine (MET) has been shown to be a tumor-selective therapeutic target for cancer, since cancer cells require higher amounts of MET to divide and survive than normal cells. This phenomena is known as MET dependence and is probably due to MET overuse by cancer cells. A pilot clinical trial was initially carried out with non-recombinant METase (METase) produced from Pseudomonas putida and subsequently highly purified. No acute clinical toxicity was observed for any criteria measured in the three patients. The depletion of serum MET started within 30 min of the infusion and was maintained for 4 h after the infusion was completed in patient 1 and patient 2. The lowest serum MET levels were 35% and 19% of the pretreatment level, respectively, in patient 1 and patient 2. Patient 3 received a 10 h i.v. infusion of METase without any sign of side effects. MET was depleted over 200-fold from 23.1 to 0.1 μM by the 10-h infusion of patient 3. No clinical toxicity was observed in any criteria measured in patient 3. Subsequently, another pilot Phase I clinical trial was carried out of serum MET depletion in cancer patients by recombinant METase (rMETase) cloned from Pseudomonas putida and produced in E. coli. Patients with advanced breast cancer, lung cancer, renal cancer, and lymphoma were given a single rMETase treatment at doses ranging from 5000 to 20,000 units by i.v. infusion over 6–24 h. No clinical toxicity was observed in any patient after rMETase treatment. rMETase levels were measured at 0.1 to 0.4 units per ml of serum in the patients which correspond to therapeutic levels in vitro. The lowest serum MET levels in rMETase-treated patients were 0.1% of the pretreatment levels corresponding to approximately 0.1 μM, which also correlates to therapeutic levels in vitro as well as in vivo. The results of the METase and rMETase pilot Phase I clinical trials therefore indicate that i.v. infusion of rMETase is safe and effectively depletes its biochemical target of serum MET, suggesting potential efficacy in future clinical trials.

Key words

Methionine (MET) dependence Methioninase Advanced cancer patient High-stage Clinical trial MET restriction 

References

  1. 1.
    Hoffman RM (2015) Development of recombinant methioninase to target the general cancer-specific metabolic defect of methionine dependence: a 40-year odyssey. Expert Opin Biol Ther 15:21–31CrossRefGoogle Scholar
  2. 2.
    Hoffman RM, Jacobsen SJ (1980) Reversible growth arrest in SV40-transformed human fibroblasts. Proc Natl Acad Sci U S A 77:7306–7310CrossRefGoogle Scholar
  3. 3.
    Guo H, Lishko K, Herrera H, Groce A, Kubota T, Hoffman RM (1993) Therapeutic tumor-specific cell cycle block induced by methioninase starvation in vivo. Cancer Res 53:5676–5679Google Scholar
  4. 4.
    Tan Y, Zavala J Sr, Xu M, Zavala J Jr, Hoffman RM (1996) Serum MET depletion without side effects by METase in metastatic breast cancer patients. Anticancer Res 16:3937–3942PubMedPubMedCentralGoogle Scholar
  5. 5.
    Goseki N, Yamazaki S, Shimojyu K, Kando F, Maruyama M, Endo M, Koike M, Takahashi H (1995) Synergistic effect of methionine-depleting total parenteral nutrition with 5-fluorouracil on human gastric cancer. A randomized, prospective clinical trial. Jpn J Cancer Res 86:484–489CrossRefGoogle Scholar
  6. 6.
    Goseki N, Yamazaki S, Endo M (1992) Antitumor effect of methionine-depleting total parenteral nutrition with doxorubicin administration on Yoshida sarcoma-bearing rate. Cancer 69:1865–1872CrossRefGoogle Scholar
  7. 7.
    Tan Y, Zavala J Sr, Han Q, Xu M, Sun X, Tan X-Z, Tan X-Y, Magana R, Geller J, Hoffman RM (1997) Recombinant METase infusion reduces the biochemical endpoint of serum MET with minimal toxicity in high-stage cancer patients. Anticancer Res 17:3857–3860PubMedPubMedCentralGoogle Scholar
  8. 8.
    Lishko VK, Lishko OV, Hoffman RM (1993) The preparation of endotoxin-free L-methionine-α-deamino-γ-mercaptomethane-lyase (L-methioninase) from Pseudomonas putida. Protein Expr Purif 4:529–533CrossRefGoogle Scholar
  9. 9.
    Tan Y, Xu M, Sun X, Kubota T, Hoffman RM (1996) Anticancer efficacy of methioninase in vivo. Anticancer Res 16:3931–3936Google Scholar
  10. 10.
    Tan Y, Xu M, Tan XZ, Tan X, Wang X, Saikawa S, Nagahama T, Sun X, Lenz M, Hoffman RM (1997) Overexpression and large-scale production of recombinant L-methionine-α-deamino-γ-mercaptomethane-lyase for novel anticancer therapy. Protein Expr Purif 9:233–245CrossRefGoogle Scholar
  11. 11.
    Tan Y, Sun X, Xu M, Tan X-Z, Sasson A, Rashidi B, Han Q, Tan X-Y, Wang X, An Z, Sun F-X, Hoffman RM (1999) Efficacy of recombinant methioninase in combination with cisplatin on human colon tumors in nude mice. Clin Cancer Res 5:2157–2163Google Scholar
  12. 12.
    Tan Y, Xu M, Hoffman RM (2010) Broad selective efficacy of recombinant methioninase and polyethylene glycol-modified recombinant methioninase on cancer cells in vitro. Anticancer Res 30:1041–1046Google Scholar
  13. 13.
    Jones BN, Gilligan JP (1983) α-Phthaldialdehyde precolumn derivatization and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids. J Chromatogr 266:471–482CrossRefGoogle Scholar
  14. 14.
    Guidelines for reporting of adverse drug reactions (1985) Division of Cancer Treatment, National Cancer Institute, Bethesda, MDGoogle Scholar
  15. 15.
    Tan Y, Han O, An Z, Wang X, Hoffman RM (1995) Methioninase (AC 9301): a selective antitumor agent with a new mechanism of action. Proc Am Soc Clin Oncol 14:493Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Robert M. Hoffman
    • 1
    • 2
    Email author
  • Yuying Tan
    • 1
  • Shukuan Li
    • 1
  • Qinghong Han
    • 1
  • Jorge ZavalaSr
    • 3
  • Jorge ZavalaJr
    • 3
  1. 1.AntiCancer, Inc.San DiegoUSA
  2. 2.Department of SurgeryUniversity of CaliforniaSan DiegoUSA
  3. 3.Comprehensive Cancer Center of Northwest MexicoTijuanaMexico

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