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Xenopus pp 19-32 | Cite as

Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos

  • Marcin Wlizla
  • Sean McNamara
  • Marko E. Horb
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1865)

Abstract

Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols. Here, we discuss the procedures used at the National Xenopus Resource, which we found helpful for generation and early upkeep of Xenopus embryos and tadpoles.

Key words

Xenopus laevis Xenopus tropicalis In vitro fertilization hCG oLH 

Notes

Acknowledgments

The National Xenopus Resource is supported by a grant from the National Institutes of Health (P40 OD010997).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.National Xenopus ResourceMarine Biological LaboratoryWoods HoleUSA

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