Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos
Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols. Here, we discuss the procedures used at the National Xenopus Resource, which we found helpful for generation and early upkeep of Xenopus embryos and tadpoles.
Key wordsXenopus laevis Xenopus tropicalis In vitro fertilization hCG oLH
The National Xenopus Resource is supported by a grant from the National Institutes of Health (P40 OD010997).