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Protocol for Agrobacterium-Mediated Transformation and Transgenic Plant Production of Switchgrass

  • QiuXia Chen
  • Guo-Qing Song
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Switchgrass (Panicum virgatum L.) is one of the most important bioenergy crops for lignocellulose ethanol production. Molecular breeding provides a powerful tool to supplement conventional switchgrass breeding by introducing or editing genes of interest. In this chapter, we describe Agrobacterium tumefaciens-mediated transformation protocols for lowland tetraploid switchgrass cultivar Alamo.

Key words

Agrobacterium tumefaciens Genetic transformation Plant regeneration Somatic embryogenesis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Horticulture, Plant Biotechnology Resource and Outreach CenterMichigan State UniversityEast LansingUSA

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