Transient Transformation Using Particle Bombardment for Gene Expression Analysis
Transient transformation or transient expression results in rapid and fleeting gene expression. This approach is often used as a first-tier screening tool for evaluation of components that affect gene expression. Here, we describe the use of particle bombardment of lima bean cotyledons with constructs containing the green fluorescent protein (gfp) coding region for evaluation of promoter components that influence gene expression. Although this approach is conceptually quite simple, this lima bean transient expression system may not work well, if our methods and notes are not carefully read and followed. Our laboratory has successfully optimized this method over the past 10 years, resulting in a transient expression system, which works like no other that we have seen.
Key wordsPromoter analysis Transient expression Gene introduction Green fluorescent protein Lima bean cotyledon
Salaries and research support were provided by the United Soybean Board and by state and federal funds appropriated to the Ohio State University/Ohio Agricultural Research and Development Center. Mention of trademark or proprietary products does not constitute a guarantee or warranty of the product and also does not imply approval to the exclusion of other products that may also be suitable.