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Ensifer-Mediated Transformation (EMT) of Rice (Monocot) and Oilseed Rape (Dicot)

  • Dheeraj Singh Rathore
  • Evelyn Zuniga-Soto
  • Ewen Mullins
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Ensifer adhaerens OV14 underpins the successful crop transformation protocol, termed Ensifer-mediated transformation (EMT). The adaptability and efficiency of EMT technology to successfully transform both monocot and dicots have been previously reported. To facilitate community users’ transition to EMT, the modified rice and oilseed rape plants generated in this work were developed using EMT protocols that were grounded in standard Agrobacterium-mediated transformation (AMT) processes. Therefore, this chapter describes simple yet crucial steps involved in transferring the use of EMT of rice and oilseed rape for generation of fertile and independent transgenic lines.

Key words

Ensifer adhaerens Transformation Monocot Dicot Plant 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Dheeraj Singh Rathore
    • 1
  • Evelyn Zuniga-Soto
    • 1
  • Ewen Mullins
    • 1
  1. 1.Department of Crop Science, TeagascCarlow R93 XE12Republic of Ireland

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