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Detection of Transgenic Proteins by Immunoassays

  • Satyalinga Srinivas Gampala
  • Bryant Wulfkuhle
  • Kimberly A. Richey
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Rapid development and global cultivation of genetically modified (GM) crops necessitated the use of analytical tools that detect GM crops throughout the product life cycle. Antibody-based immunoassays such as enzyme-linked immunosorbent assays (ELISA) are instrumental in detecting protein expression in transgenic plants. These analytical tools are used throughout development, regulatory registration, commercialization, and stewardship of biotech products. Here we describe the Cry1F ELISA analytical method protocol, data generation and review, and troubleshooting of technical challenges.

Key words

Monoclonal and polyclonal antibodies Immunoassays Sandwich ELISA Transgene expression Transgenic proteins Genetically modified (GM) crops Cry1F 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Satyalinga Srinivas Gampala
    • 1
  • Bryant Wulfkuhle
    • 1
  • Kimberly A. Richey
    • 1
  1. 1.Corteva Agriscience™Agriculture Division of DowDuPont™IndianapolisUSA

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