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Transgenic and Genome Editing Approaches for Modifying Plant Oils

  • Laura L. Wayne
  • Daniel J. Gachotte
  • Terence A. Walsh
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Vegetable oils are important for human and animal nutrition and as renewable resources for chemical feedstocks. We provide an overview of transgenic and genome editing approaches for modifying plant oils, describing useful model and crop systems and different strategies for transgenic modifications. We also describe new genome editing approaches that are beginning to be applied to oilseed plants and crops. These approaches are illustrated with examples for modifying the nutritional quality of vegetable oils by altering fatty acid desaturation, producing non-native fatty acids in oilseeds, and enhancing the overall accumulation of oil in seeds and leaves.

Key words

Lipid biosynthesis Metabolic engineering Fatty acid Polyunsaturated fatty acids (PUFAs) Healthy oils Industrial oils CRISPR Genome editing Fatty acid desaturase Acyl transferase 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Laura L. Wayne
    • 1
  • Daniel J. Gachotte
    • 2
  • Terence A. Walsh
    • 2
  1. 1.Corteva Agriscience™Agriculture Division of DowDuPont™JohnstonUSA
  2. 2.Corteva Agriscience™Agriculture Division of DowDuPont™IndianapolisUSA

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