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Agrobacterium-Mediated Transformation of Solanum tuberosum L., Potato

  • Myron A. Bruce
  • Jessica L. Shoup Rupp
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Potato is considered the fourth most important food crop in the world, and the most important non-cereal crop. Potato is transformed using Agrobacterium tumefaciens with relative ease. Several improvements have been made in the last 20 years with respect to tissue culture, transformation, and regeneration of potato. This chapter describes a reliable and efficient potato transformation system using internodal explants. Plasmid preparation, Agrobacterium transformation, potato transformation, regeneration, and recovery are described in detail, as well as molecular characterization of resulting putative transgenic plants.

Key words

Potato Solanum tuberosum L. Internodal explant Tuber Antibiotic selection 

Notes

Acknowledgments

A sincere thank you to Whitney Harchenko, for providing photos of her work in transforming potato.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Montana State UniversityBozemanUSA
  2. 2.Kansas State UniversityManhattanUSA

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