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The Genetic Transformation of Sweet Orange (Citrus sinensis L. Osbeck) for Enhanced Resistance to Citrus Canker

  • Lorena Noelia Sendin
  • María Paula Filippone
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Developing disease resistance is one of the most important components of any plant breeding program. Citrus traditional breeding methods (bud sport selection, crossbreeding, and other breeding channels) are a laborious task and often hampered by long juvenility, a high degree of heterozygosity, polyembryony, self-incompatibility, and abortion of reproductive organs. An interesting alternative to the classical breeding approach is the use of genetic transformation, which provides the means for adding a single agronomic trait to a plant without otherwise altering its phenotype. Agrobacterium tumefaciens-mediated transformation has been carried out with numerous hybrids and citrus species. This technique allowed us to introduce the Bs2 gene in Citrus, as well as to increase citrus canker resistance in transgenic Bs2 gene-expressing lines.

Key words

Citrus breeding A. tumefaciens Shoot regeneration In vitro grafting Acclimatization 

Notes

Acknowledgments

This project was supported by Estación Experimental Agroindustrial Obispo Colombres (EEAOC) and Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Lorena Noelia Sendin
    • 1
  • María Paula Filippone
    • 1
  1. 1.Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), EEAOC-CONICETLas TalitasArgentina

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