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Repurposing Macromolecule Delivery Tools for Plant Genetic Modification in the Era of Precision Genome Engineering

  • Qiudeng Que
  • Mary-Dell M. Chilton
  • Sivamani Elumalai
  • Heng Zhong
  • Shujie Dong
  • Liang Shi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Efficient delivery of macromolecules into plant cells and tissues is important for both basic research and biotechnology product applications. In transgenic research, the goal is to deliver DNA molecules into regenerable cells and stably integrate them into the genome. Over the past 40 years, many macromolecule delivery methods have been studied. To generate transgenic plants, particle bombardment and Agrobacterium-mediated transformation are the methods of choice for DNA delivery. The rapid advance of genome editing technologies has generated new requirements on large biomolecule delivery and at the same time reinvigorated the development of new transformation technologies. Many of the gene delivery options that have been studied before are now being repurposed for delivering genome editing machinery for various applications. This article reviews the major progress in the development of tools for large biomolecule delivery into plant cells in the new era of precision genome engineering.

Key words

Direct delivery Biolistic transformation Agrobacterium-mediated transformation Protoplast transformation Macromolecule delivery DNA-free delivery Cell-penetrating peptides Genome engineering 

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Qiudeng Que
    • 1
  • Mary-Dell M. Chilton
    • 1
  • Sivamani Elumalai
    • 1
  • Heng Zhong
    • 1
  • Shujie Dong
    • 1
  • Liang Shi
    • 1
  1. 1.Syngenta Crop Protection, LLCResearch Triangle ParkUSA

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