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Isolation of High Molecular Weight DNA from Insects

  • Susan J. Brown
  • Michelle Coleman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1858)

Abstract

Isolation of high molecular weight (HMW) DNA from any organism can be daunting and populations of extremely small insects with hard exoskeletons are no exception. It can be challenging to isolate DNA that is clean enough to enzymatically label, digest or otherwise manipulate without sacrificing length. The method described here was originally developed to isolate HMW DNA for BAC library construction. More recently, it has proven reliable for long range optical mapping as well.

Key words

High molecular weight DNA Optical mapping Long range restriction mapping 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of BiologyKansas State UniversityManhattanUSA

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