LiveFly: A Toolbox for the Analysis of Transcription Dynamics in Live Drosophila Embryos
We present the LiveFly toolbox for quantitative analysis of transcription dynamics in live Drosophila embryos. The toolbox allows users to process two-color 3D confocal movies acquired using nuclei-labeling and the fluorescent RNA-tagging system described in the previous chapter and export the nuclei’s position as a function of time, their lineages and the intensity traces of the active loci. The toolbox, which is tailored for the context of Drosophila early development, is semiautomatic, and requires minimal user intervention. It also includes a tool to combine data from multiple movies and visualize several features of the intensity traces and the expression pattern.
Key wordsDrosophila melanogaster Real-time monitoring Transcription dynamics Image analysis Early development
This work was supported by a Marie Curie MCCIG grant No. 303561 (AMW), PSL IDEX REFLEX (ND, AMW, MC), ANR-11-LABX-0044 DEEP Labex (ND), ANR- 11-BSV2-0024 Axomorph (ND and AMW), and PSL ANR-10-IDEX-0001-02. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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