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Measuring In Vivo Tissue Metabolism Using 13C Glucose Infusions in Mice

  • Dorien Broekaert
  • Sarah-Maria Fendt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)

Abstract

Metabolic alterations are a hallmark of cancer. While determining metabolic changes in vitro has delivered valuable insight into the metabolism of cancer cells, it emerges that determining the in vivo metabolism adds an additional layer of information. Here, we therefore describe how to measure the in vivo metabolism of cancer tissue using 13C glucose infusions in mice.

Key words

In vivo metabolism 13C tracer analysis Metabolomics 13C glucose infusions Cancer metabolism 

Notes

Acknowledgments

We thank Juan Fernández-García for his feedback on the protocol. S.-M.F. acknowledges funding from the European Research Council under the ERC Consolidator Grant Agreement n. 771486–MetaRegulation; Marie Curie—CIG, FWO—Odysseus II, FWO—Research Grants/Projects, Eugène Yourassowsky Schenking, and KU Leuven—Methusalem Co-Funding. We would like to acknowledge http://www.somersault1824.com for image elements used in Fig. 6 (Creative Commons license CC BY-NC-SA 4.0).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer BiologyVIBLeuvenBelgium
  2. 2.Laboratory of Cellular Metabolism and Metabolic Regulation, Department of Oncology, Leuven Cancer Institute (LKI)KU LeuvenLeuvenBelgium
  3. 3.Laboratory of Cellular Metabolism and Metabolic Regulation, Department of OncologyVIB-KU Leuven Center for Cancer BiologyLeuvenBelgium

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