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Lipectomizing Mice for Applications in Metabolism

  • Debrup Chakraborty
  • Jamie J. Bernard
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)

Abstract

The obesity epidemic is a critical public health problem closely associated with the development of metabolic disease. In obesity there is excess white adipose tissue, a dynamic tissue that has many biological functions. Specifically visceral adipose tissue (VAT) is an active endocrine organ producing hormones that control systemic metabolism. VAT accumulates immune cells that produce cytokines that drive chronic inflammation and promote insulin resistance. VAT can be surgically removed in experimental animals (lipectomy) to explore mechanisms by which VAT participates in metabolic, endocrine, and immunological functions. This chapter describes the technical protocol for efficient and successful removal of the gonadal fat pads in mice.

Key words

Lipectomy Visceral adipose tissue Mouse surgery Metabolism High-fat diet Obesity 

Notes

Conflict of Interest Statement

The authors declare no conflict of interest.

Support for Work

National Institutes of Health grant R00 CA177868, Michigan State University start-up funds

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pharmacology and ToxicologyMichigan State UniversityEast LansingUSA

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