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Development of Patient-Derived Tumor Xenograft Models

  • Daniela Annibali
  • Eleonora Leucci
  • Els Hermans
  • Frédéric Amant
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)

Abstract

In spite of the latest advancements in understanding cancer development and progression, drugs successful in preclinical testing often fail upon reaching phase III clinical trials. A reason for this is the use of inappropriate preclinical models that do not preserve tumor heterogeneity. Although used for decades, cell cultures derived from patients substantially deviate from their original biopsy upon culturing; moreover, they cannot predict the response of an organism as a whole.

Patient-derived xenograft (PDX) models are emerging as powerful tools since they have a predictive therapeutic value and preserve the heterogeneity of the original tumors. PDX are established by implanting freshly isolated tumors from patients into immunocompromised mice, allowing for the progressive growth and amplification of cancer tissue for in vivo testing. Here, we describe the detailed methods we developed to establish PDX from both surgically removed endometrial cancer fragments (endometrial cancer) and fine-needle aspiration biopsies (pancreatic cancer).

Key words

PDX Tumor models In vivo Xenografts 

Notes

Acknowledgment

Trace, the KU Leuven PDX Platform has been established thanks to grants from the National Kanker Plan (NKP), Belgium, and the Verelst Uterine Cancer Fund Leuven (VBL). Trace also acknowledges the support of the Stichting tegen Kanker Foundation and is part of the EuroPDX Consortium. FA is a senior researcher for the Research Fund Flanders (F.W.O.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Daniela Annibali
    • 1
  • Eleonora Leucci
    • 2
    • 3
  • Els Hermans
    • 2
    • 4
  • Frédéric Amant
    • 1
    • 5
  1. 1.Gynecological Oncology, Oncology DepartmentLKI Leuven Cancer Institute KU Leuven-University of LeuvenLeuvenBelgium
  2. 2.Trace, Department of OncologyLKI Leuven Cancer Institute KU Leuven-University of LeuvenLeuvenBelgium
  3. 3.Laboratory of RNA Cancer Biology, Department of OncologyLKI Leuven Cancer Institute KU Leuven-University of LeuvenLeuvenBelgium
  4. 4.VIB-KU Leuven Center for Cancer BiologyLeuvenBelgium
  5. 5.Centre for Gynecologic Oncology Amsterdam (CGOA)Antoni Van Leeuwenhoek-Netherlads Cancer Institute (AvL-NKI) and University Medical Centra (UMC)AmsterdamThe Netherlands

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