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Assessing the Impact of the Nutrient Microenvironment on the Metabolism of Effector CD8+ T Cells

  • Juan Fernández-García
  • Sarah-Maria Fendt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)

Abstract

Immune cell function is tightly regulated by cellular metabolism, which in turn is strongly linked to the nutrient availability in the microenvironment surrounding the cells. This link is critical for effector CD8+ T cells which, after activation, must migrate from nutrient-rich environments into nutrient-scarce regions such as the tumor microenvironment. Assessing how nutrient availability modulates the metabolism of effector CD8+ T cells is thus key for understanding how harsh environments may impair their proliferation and effector function. Here, we describe an approach to systematically study the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells, based on performing stable 13C isotope labeling measurements on in vitro-differentiated murine effector CD8+ T cells.

Key words

CD8+ T cells Immunometabolism 13C tracer analysis Nutrient microenvironment Custom media formulations 

Notes

Acknowledgments

JFG is supported by an FWO (Fonds voor Wetenschappelijk Onderzoek–Vlaanderen, Research Foundation–Flanders) Postdoctoral Fellowship. S-MF acknowledges funding from the European Research Council under the ERC Consolidator Grant Agreement no. 771486 – MetaRegulation, Marie Curie – CIG, FWO – Odysseus II, FWO – Research Grants/Projects, Eugène Yourassowsky Schenking, and KU Leuven – Methusalem Co-Funding. We would like to acknowledge http://www.somersault1824.com for image elements used in Fig. 1 (Creative Commons license CC BY-NC-SA 4.0).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer BiologyVIBLeuvenBelgium
  2. 2.Laboratory of Cellular Metabolism and Metabolic Regulation, Department of Oncology, Leuven Cancer Institute (LKI)KU LeuvenLeuvenBelgium
  3. 3.Laboratory of Cellular Metabolism and Metabolic Regulation, Department of OncologyVIB-KU Leuven Center for Cancer BiologyLeuvenBelgium

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