Live Imaging of Xist RNA
Live imaging gives additional layers of information such as physical dynamics of a molecule of your interest. Aptamer-based green fluorescent protein (GFP) labeling is suitable for visualization of RNA molecules. Here we describe a method to visualize Xist RNA using the Bgl aptamer system.
Key wordsLive imaging Aptamer Xist RNA Bgl system
I thank Drs. Edith Heard and Haruhiko Koseki for critical discussions. This work was supported by PRESTO and KAKENHI to O.M.