Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry
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Capillary electrophoresis coupled to mass spectrometry is an analytical platform ideal for the analysis of ionic or polar metabolites. It constitutes a perfect complement to reversed-phase liquid chromatography, offering a good alternative to polar stationary phases where reproducibility is not guaranteed. Herein, we describe a robust standardized methodology for the fingerprinting analysis of Leishmania, a taxonomic genus which comprises more than 20 protozoa species.
Key wordsFingerprinting Parasite phenotyping Polar metabolites Untargeted metabolomics
This work has been supported by the funding from the Spanish Ministry of Economy and Competitiveness (CTQ2014-55279-R).
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