Detection of Phosphoproteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using 8-Quinolinol Stain
In order to detect phosphoproteins in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), an easy and fast fluorescent detection method is described. 8-Quinolinol can form ternary complexes in the gel matrix contributed by the affinity of aluminum ion to the phosphate groups on the proteins and the metal chelating property of 8-Quinolinol, exhibiting strong fluorescence in ultraviolet light. It can visualize as little as 4–8 ng of α-casein and β-casein, 15–31 ng of ovalbumin and κ-casein within 70 min. The approach utilizing 8-quinolinol could be an alternative staining method for phosphoproteomics.
Key words8-Quinolinol Aluminum ion Fluorescent detection Phosphoprotein stain SDS-PAGE
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A3B03932150).