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Detection of Phosphoproteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using 8-Quinolinol Stain

  • Sun-Young Hwang
  • Xu Wang
  • Jung-Kap Choi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

In order to detect phosphoproteins in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), an easy and fast fluorescent detection method is described. 8-Quinolinol can form ternary complexes in the gel matrix contributed by the affinity of aluminum ion to the phosphate groups on the proteins and the metal chelating property of 8-Quinolinol, exhibiting strong fluorescence in ultraviolet light. It can visualize as little as 4–8 ng of α-casein and β-casein, 15–31 ng of ovalbumin and κ-casein within 70 min. The approach utilizing 8-quinolinol could be an alternative staining method for phosphoproteomics.

Key words

8-Quinolinol Aluminum ion Fluorescent detection Phosphoprotein stain SDS-PAGE 

Notes

Acknowledgment

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A3B03932150).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Analytical Biochemistry, College of Pharmacy & Research Institute of Drug DevelopmentChonnam National UniversityGwangjuSouth Korea
  2. 2.School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical EngineeringWenzhou Medical UniversityWenzhouP. R. China

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