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Silver Staining Techniques of Polyacrylamide Gels

  • Nicole Berndt
  • Ralf Bergmann
  • Claudia Arndt
  • Stefanie Koristka
  • Michael Bachmann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

After SDS–polyacrylamide gel electrophoresis the separated proteins have to be visualized by staining the gel. The same is true after transfer of separated proteins to a blotting membrane in order to verify an efficient transfer and to visualize the amount of protein(s) remaining in the gel. Several different staining techniques exist for staining of proteins in SDS–polyacrylamide gels. The sensitivity of these staining procedures are different, also the expenditure of time and other aspects. Still, silver staining is among the most sensitive and reliable staining technique. Because this technique was developed in the 1970s, a huge number of variations exist. Here, we will provide three variations, which are robust and easy to perform.

Key words

Silver staining Polyacrylamide gels Proteins 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nicole Berndt
    • 1
  • Ralf Bergmann
    • 1
  • Claudia Arndt
    • 1
  • Stefanie Koristka
    • 1
  • Michael Bachmann
    • 1
  1. 1.Institute of Radiopharmaceutical Cancer Research, Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf e.V. (HZDR)DresdenGermany

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