A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Using Coomassie Blue

  • Wei-hua Dong
  • Fang Wang
  • Jun-he Zhang
  • Yan-sheng Zhou
  • Ling-ye Zhang
  • Tian-yun Wang
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)


Most traditional post-electrophoretic processes need several hours to several days to finish the whole staining process and traditional staining solutions all contain methanol, acetic acid, or phosphoric acid, which not only produce the unpleasant smell but also cause environmental pollution. Here a fixation-free, fast protein staining method in sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol includes only staining and quick washing steps, can be completed in 0.5 h. It has a sensitivity of 10 ng. In addition, the dye stain does not contain any acid or methanol.

Key words

CBB R-250 Protein staining PAGE Simple Time-saving 



This work was supported by the grants from National Natural Science Foundation of China (No. 81673337).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Wei-hua Dong
    • 1
  • Fang Wang
    • 1
  • Jun-he Zhang
    • 1
  • Yan-sheng Zhou
    • 1
  • Ling-ye Zhang
    • 1
  • Tian-yun Wang
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyXinxiang Medical UniversityHenanChina

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