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Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate–Polyacrylamide Protein Gels Using a Household Detergent

  • Rachna Aggarwal
  • Biji T. Kurien
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

We report here a one-step method using the household detergent Vim Ultra to destain sodium dodecyl sulfate–polyacrylamide protein gels stained with Coomassie Brilliant Blue. This method, originally described by Pal and group, uses a 5% suspension of the detergent to destain gels efficiently. This method is cheap and user-friendly compared to the commonly used methanol–acetic acid–water containing destaining solvent.

Key words

SDS-PAGE Coomassie stain Detergent Vim Ultra Destaining 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Arthritis and Clinical ImmunologyOklahoma Medical Research Foundation, University of OklahomaOklahoma CityUSA
  2. 2.Section of Endocrinology and DiabetesUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  3. 3.Department of Veterans Affairs Medical CenterOklahoma CityUSA
  4. 4.Department of Arthritis and Clinical ImmunologyOklahoma CityUSA

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