Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate–Polyacrylamide Protein Gels Using a Household Detergent

  • Rachna Aggarwal
  • Biji T. KurienEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)


We report here a one-step method using the household detergent Vim Ultra to destain sodium dodecyl sulfate–polyacrylamide protein gels stained with Coomassie Brilliant Blue. This method, originally described by Pal and group, uses a 5% suspension of the detergent to destain gels efficiently. This method is cheap and user-friendly compared to the commonly used methanol–acetic acid–water containing destaining solvent.

Key words

SDS-PAGE Coomassie stain Detergent Vim Ultra Destaining 


  1. 1.
    Wu W, Welsh MJ (1996) Rapid Coomassie blue staining and destaining of polyacrylamide gels. BioTechniques 20:386–388CrossRefPubMedGoogle Scholar
  2. 2.
    Kurien BT, Scofield RH (1998) Heat mediated quick Coomassie Blue protein staining and destaining of SDS-PAGE gels. Indian J Biochem Biophys 35:385–389PubMedGoogle Scholar
  3. 3.
    Ornstein L (1959) Simultaneous preservation of intracellular morphology and enzymatic or antigenic activities in frozen tissues for high resolution histochemistry. J Histochem Cytochem 7:291 In: published discussion of Davis, B. J., L. Ornstein, P. Taleporos and S. Koulish. 1959Google Scholar
  4. 4.
    Raymond S, Weintraub L (1959) Acrylamide gel as a supporting medium for zone electrophoresis. Science 130:711CrossRefPubMedGoogle Scholar
  5. 5.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefGoogle Scholar
  6. 6.
    Dorri Y, Kurien BT (2010) Environmentally safe removal/disposal of Coomassie Brilliant Blue from gel destain and used gel stain. Anal Biochem 404:193–196CrossRefPubMedGoogle Scholar
  7. 7.
    Pal JK, Sarkar A, Katoch B (2001) Detergent-mediated destaining of Coomassie Brilliant Blue-stained SDS polyacrylamide gels. Indian J Exp Biol 39:95–97PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Arthritis and Clinical ImmunologyOklahoma Medical Research Foundation, University of OklahomaOklahoma CityUSA
  2. 2.Section of Endocrinology and DiabetesUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  3. 3.Department of Veterans Affairs Medical CenterOklahoma CityUSA
  4. 4.Department of Arthritis and Clinical ImmunologyOklahoma CityUSA

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