Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate–Polyacrylamide Protein Gels Using a Household Detergent
We report here a one-step method using the household detergent Vim Ultra to destain sodium dodecyl sulfate–polyacrylamide protein gels stained with Coomassie Brilliant Blue. This method, originally described by Pal and group, uses a 5% suspension of the detergent to destain gels efficiently. This method is cheap and user-friendly compared to the commonly used methanol–acetic acid–water containing destaining solvent.
Key wordsSDS-PAGE Coomassie stain Detergent Vim Ultra Destaining
- 3.Ornstein L (1959) Simultaneous preservation of intracellular morphology and enzymatic or antigenic activities in frozen tissues for high resolution histochemistry. J Histochem Cytochem 7:291 In: published discussion of Davis, B. J., L. Ornstein, P. Taleporos and S. Koulish. 1959Google Scholar