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Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain

  • Padmaja Mehta-D’souza
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic-acid Schiff’s base which uses acidic fuchsin dye.

Key words

SDS-PAGE Glycoprotein staining P-Selectin glycoprotein ligand-1 (PSGL-1) 

Notes

Acknowledgments

The author thanks Dr. Charles Esmon for the HPC4 antibody and Dr. Rodger P. McEver for purified soluble PSGL-1.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Cardiovascular Biology Research ProgramOklahoma Medical Research FoundationOklahoma CityUSA

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