Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain
Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic-acid Schiff’s base which uses acidic fuchsin dye.
Key wordsSDS-PAGE Glycoprotein staining P-Selectin glycoprotein ligand-1 (PSGL-1)
The author thanks Dr. Charles Esmon for the HPC4 antibody and Dr. Rodger P. McEver for purified soluble PSGL-1.
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