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Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-Producing Bacteria

  • Dalal Asker
  • Tarek S. Awad
  • Teruhiko Beppu
  • Kenji Ueda
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1852)

Abstract

Carotenoids are naturally occurring yellow to red pigments with many biological activities including antioxidant, anticancer, anti-inflammatory, membrane stabilizers, and precursors for vitamin A. These biological activities are linked with many health benefits (e.g., anticarcinogenic activity, prevention of chronic diseases, etc.), which grew the interest of several industrial sectors especially in food, feed, nutraceuticals, cosmetics, and pharmaceutical industries. The production of natural carotenoids from microbial sources such as bacteria can help meet the growing global market of carotenoids estimated at $1.5 billion in 2014 and is expected to reach 1.8 billion in 2019. This chapter demonstrates, step-by-step, the development of a rapid and selective screening method for isolation and identification of carotenoid-producing microorganisms and their carotenoid analysis. This method involves three main procedures: UV treatment, sequencing analysis of 16S rRNA genes, and carotenoids analysis using rapid and effective HPLC-diode array-MS methods.

Key words

Carotenoids Irradiation Misasa Bacteria Radiotolerant 16S rRNA genes sequencing analysis Phylogeny Diversity Photodiode array detection HPLC-DAD HPLC-MS 

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Authors and Affiliations

  • Dalal Asker
    • 1
    • 2
  • Tarek S. Awad
    • 2
  • Teruhiko Beppu
    • 3
  • Kenji Ueda
    • 3
  1. 1.Food Science and Technology Department, Faculty of AgricultureAlexandria UniversityAlexandriaEgypt
  2. 2.Department of Materials Science and EngineeringUniversity of TorontoTorontoCanada
  3. 3.Life Science Research Center, College of Bioresource SciencesNihon UniversityFujisawaJapan

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