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Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae

  • Yixi Su
  • Zhiqian Yi
  • Snædís Huld Björnsdóttir
  • Sigurdur Brynjolfsson
  • Weiqi Fu
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1852)

Abstract

In order to produce natural pigments with competitive prices, algal strains employed in industrial production need to be improved for increasing the productivity of valuable metabolites, thereby reducing the overall production cost. Adaptive laboratory evolution (ALE) is a traditional method for strain improvement, which has been effectively utilized in bacteria and fungi. With the growing interest in algal biotechnology, attempts have recently been put forward to improve microalgal strains with ALE approach. This chapter describes a stepwise adaptive evolution strategy that enhances carotenoid yield from microalgae.

Key words

Carotenoids Adaptive laboratory evolution (ALE) Abiotic stress Green algae Response surface methodology (RSM) Dunaliella salina 

Notes

Acknowledgment

This work was supported by Icelandic Technology Development Fund with a grant number of 163922-0611.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yixi Su
    • 1
  • Zhiqian Yi
    • 1
  • Snædís Huld Björnsdóttir
    • 1
  • Sigurdur Brynjolfsson
    • 1
  • Weiqi Fu
    • 1
  1. 1.Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural SciencesUniversity of IcelandReykjavíkIceland

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