Abstract
Clathrin-coated pits (ccp) are important structures that cells use for internalizing materials and regulating plasma membrane homeostasis. We had previously described an assay of reconstituting ccp assembly on sheets of basal plasma membranes. Here, we describe a workflow to adapt this system for monitoring the assembly of ccps over time using total internal reflection fluorescence (TIRF) microscopy.
The original version of this chapter was revised. A correction to this chapter can be found at https://doi.org/10.1007/978-1-4939-8719-1_19
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Change history
24 October 2018
This book was inadvertently published with the incorrect title as Clathrin-Mediated Endoytosis: Methods and Protocols. This has now been corrected throughout the book to Clathrin-Mediated Endocytosis: Methods and Protocols.
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Acknowledgment
This work was supported by the National Research Foundation (NRF) Singapore under its NRF Fellowship Program (M.W., NRF Award No: NRF-NRFF2011-09).
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Yong, J., Chen, Y., Wu, M. (2018). Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System. In: Swan, L. (eds) Clathrin-Mediated Endocytosis. Methods in Molecular Biology, vol 1847. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8719-1_13
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DOI: https://doi.org/10.1007/978-1-4939-8719-1_13
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