Identification and Characterization of Hair Follicle Stem Cells

  • Xiaoyang Wang
  • Yizhan Xing
  • Yuhong Li
Part of the Methods in Molecular Biology book series (MIMB, volume 1842)


Hair follicle stem cells (HFSCs) are epithelial cells that inhabit in the bulge region of hair follicles. They govern development of hair follicle as well as periodically regeneration of hair follicle. Under special condition, they also play roles in homeostasis of skin and other skin appendages. To characterize HFSCs in vitro, HFSCs must be isolated and cultured. In this chapter, we introduce a mechanical method to isolate HFSCs from mouse vibrissa hair follicle, and a modified method to culture isolated HFSCs. We also describe methods to characterize HFSCs, including clone formation assay and chamber graft assay.

Key words

Hair follicle stem cell Vibrissa hair follicle Cell culture Clone formation assay Chamber graft assay 



This work was supported by the National Natural Science Foundation of China (No. 81472895) and the Natural Science Foundation of Chongqing (No. cstc2015jcyjA1219).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoyang Wang
    • 1
    • 2
  • Yizhan Xing
    • 1
  • Yuhong Li
    • 1
  1. 1.Department of Cell BiologyThird Military Medical UniversityChongqingChina
  2. 2.Department of Developmental and Cell BiologyUniversity of IrvineIrvineUSA

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