Identification and Characterization of Hair Follicle Stem Cells
Hair follicle stem cells (HFSCs) are epithelial cells that inhabit in the bulge region of hair follicles. They govern development of hair follicle as well as periodically regeneration of hair follicle. Under special condition, they also play roles in homeostasis of skin and other skin appendages. To characterize HFSCs in vitro, HFSCs must be isolated and cultured. In this chapter, we introduce a mechanical method to isolate HFSCs from mouse vibrissa hair follicle, and a modified method to culture isolated HFSCs. We also describe methods to characterize HFSCs, including clone formation assay and chamber graft assay.
Key wordsHair follicle stem cell Vibrissa hair follicle Cell culture Clone formation assay Chamber graft assay
This work was supported by the National Natural Science Foundation of China (No. 81472895) and the Natural Science Foundation of Chongqing (No. cstc2015jcyjA1219).
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