Proteomic Signatures in Staphylococcus aureus
Despite all its apparent limitations proteome analysis based on two-dimensional protein gels combined with mass spectrometry is still the method of choice to study global protein synthesis activity in bacterial cells. Alterations in global protein synthesis play an important role during adaptation of bacteria to changing environmental conditions which are rather the role than the exception in their natural habitats. The protein synthesis pattern in response to a certain stimulus is highly specific and reflects the new challenges the bacterium has to meet. Here we present the techniques to analyze global protein synthesis in bacteria as exemplified by Staphylococcus aureus which is an important human pathogen and one main cause of nosocomial infections with severe outcome.
Key words2D PAGE [35S]-l-methionine pulse labeling Proteomic signatures Staphylococcus aureus Expression database
We are very grateful to our former colleagues at the Institute for Microbiology of the Greifswald University who were involved in these experiments. Furthermore, we thank the Decodon GmbH (Greifswald) for providing the Delta2D software. This work was supported by grants from the Bildungsministerium für Bildung und Forschung (InfektionsGenomik) and the Deutsche Forschungsgemeinschaft (SFB-TRR34, FOR 585, GRK840).
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