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Proteomic Signatures in Staphylococcus aureus

  • Susanne Engelmann
  • Stephan Fuchs
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1841)

Abstract

Despite all its apparent limitations proteome analysis based on two-dimensional protein gels combined with mass spectrometry is still the method of choice to study global protein synthesis activity in bacterial cells. Alterations in global protein synthesis play an important role during adaptation of bacteria to changing environmental conditions which are rather the role than the exception in their natural habitats. The protein synthesis pattern in response to a certain stimulus is highly specific and reflects the new challenges the bacterium has to meet. Here we present the techniques to analyze global protein synthesis in bacteria as exemplified by Staphylococcus aureus which is an important human pathogen and one main cause of nosocomial infections with severe outcome.

Key words

2D PAGE [35S]-l-methionine pulse labeling Proteomic signatures Staphylococcus aureus Expression database 

Notes

Acknowledgments

We are very grateful to our former colleagues at the Institute for Microbiology of the Greifswald University who were involved in these experiments. Furthermore, we thank the Decodon GmbH (Greifswald) for providing the Delta2D software. This work was supported by grants from the Bildungsministerium für Bildung und Forschung (InfektionsGenomik) and the Deutsche Forschungsgemeinschaft (SFB-TRR34, FOR 585, GRK840).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute for MicrobiologyTechnical University BraunschweigBraunschweigGermany
  2. 2.Microbial ProteomicsHelmholtz Centre for Infection ResearchBraunschweigGermany
  3. 3.Robert Koch InstituteWernigerodeGermany

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