Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganisms
OMICs-based investigations of microorganisms are becoming more and more widespread in the upcoming era of systems and synthetic biology. Here, proteomics plays a key role and two-dimensional difference gel electrophoresis (2D DIGE) remains the “gold-standard” for globally determining protein abundance changes on a quantitative and statistically confident level—in particular also for laboratories not having full-cycle proteomic facilities at their disposal. In this contribution we summarize our methodological procedures and experiences with 2D DIGE accumulated over the past 15 years.
Key words2D DIGE 2DE Fluorescence labeling Image analysis Quantitative proteome profiling Differential proteomics Microorganisms
Proteomic research (incl. DIGE) in our laboratory was supported by the German Federal Ministry of Education and Research BMBF (REGX-project), the Max Planck Society, and the Deutsche Forschungsgemeinschaft (SPP 1319, SFB TRR 51, GRK 1885).
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