Protein Enrichment from Highly Dilute Samples with StrataClean
Efficient and reproducible protein enrichment is a prerequisite for the proteomic analysis of highly dilute solutions like culture supernatant or body fluids. Traditionally several precipitation strategies were used for this purpose, but they have certain drawbacks. Here, a protocol for StrataClean enrichment—a very efficient and easy-to-use method for the enrichment of proteins from highly dilute samples—which is compatible to gel-free proteomics, is presented.
Key wordsProtein enrichment StrataClean Solid-phase enrichment Highly dilute samples
The authors like to thank Jürgen Bartel and Knut Büttner for their support in developing of the protocol. This work was supported by grants from the Deutsche Forschungsgemeinschaft (Transregio 34 Pathophysiology of staphylococci in the post-genomic era).