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Protein Enrichment from Highly Dilute Samples with StrataClean

  • Florian Bonn
  • Andreas Otto
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1841)

Abstract

Efficient and reproducible protein enrichment is a prerequisite for the proteomic analysis of highly dilute solutions like culture supernatant or body fluids. Traditionally several precipitation strategies were used for this purpose, but they have certain drawbacks. Here, a protocol for StrataClean enrichment—a very efficient and easy-to-use method for the enrichment of proteins from highly dilute samples—which is compatible to gel-free proteomics, is presented.

Key words

Protein enrichment StrataClean Solid-phase enrichment Highly dilute samples 

Notes

Acknowledgments

The authors like to thank Jürgen Bartel and Knut Büttner for their support in developing of the protocol. This work was supported by grants from the Deutsche Forschungsgemeinschaft (Transregio 34 Pathophysiology of staphylococci in the post-genomic era).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Biochemistry II, University HospitalGoethe University School of MedicineFrankfurtGermany
  2. 2.Department of Microbial Proteomics, Institute for MicrobiologyUniversity GreifswaldGreifswaldGermany

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