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Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation

  • Angel Manteca
  • Beatriz Rioseras
  • Adelina Rogowska-Wrzesinska
  • Ole N. Jensen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1841)

Abstract

The extension and biological role of Ser/Thr/Tyr phosphorylation in prokaryotes have been only scarcely studied. In this chapter, we describe the state of the art of microbial phosphoproteomics, focusing on protocols used for studying the phosphoproteome of Streptomyces coelicolor, one of the bacteria encoding the largest number of eukaryote-like Ser/Thr/Tyr kinases.

Key words

Streptomyces Phosphoproteomics Differentiation Sporulation LC-MS/MS 

Notes

Acknowledgments

We wish to thank the European Research Council (ERC Starting Grant; Strp-differentiation 280304), the Spanish “Ministerio de Economía y Competitividad” (MINECO; BIO2015-65709-R), and the VILLUM Foundation (VILLUM Center for Bioanalytical Sciences at University of Southern Denmark) for financial support.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Angel Manteca
    • 1
  • Beatriz Rioseras
    • 1
  • Adelina Rogowska-Wrzesinska
    • 2
  • Ole N. Jensen
    • 2
  1. 1.Area de Microbiologia, Departamento de Biologia Funcional, Facultad de Medicina, IUOPAUniversidad de OviedoOviedoSpain
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of Southern DenmarkOdenseDenmark

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