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Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses

  • Florian-Alexander Herbst
  • Nico Jehmlich
  • Martin von Bergen
  • Frank Schmidt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1841)

Abstract

Quantitative proteome profiling of microorganisms by isotopic labeling of amino acids is still a challenge, because only microorganisms with auxotrophic character are able to embed amino acids into their biomass in a quantitatively correct manner. Here, we describe an isotopic labeling technique (sulfur stable isotope labeling of amino acids for quantification, SULAQ) for the sulfur-containing amino acids cysteine and methionine in a broad range of organisms. The metabolic labeling approach is suitable for gel-based and gel-free protein analysis.

Key words

Sulfur stable isotope labeling of amino acids for quantification SULAQ Sulfur-34S Sulfur-36S Quantitative proteomics 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Florian-Alexander Herbst
    • 1
  • Nico Jehmlich
    • 2
  • Martin von Bergen
    • 2
    • 3
  • Frank Schmidt
    • 4
  1. 1.Department of Chemistry and BioscienceAalborg UniversityAalborg EastDenmark
  2. 2.Department of Molecular Systems BiologyHelmholtz Centre for Environmental Research–UFZLeipzigGermany
  3. 3.Department of MetabolomicsHelmholtz Centre for Environmental Research–UFZLeipzigGermany
  4. 4.Interfaculty Institute for Genetics and Functional GenomicsUniversity Medicine GreifswaldGreifswaldGermany

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