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Porcine RPE/Choroidal Explant Cultures

  • Alexa KlettnerEmail author
  • Yoko Miura
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1834)

Abstract

The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

Key words

Retinal pigment epithelium Bruch’s membrane Perfusion culture 

Notes

Acknowledgments

AK is funded by the Hermann-Wacker foundation.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Ophthalmology, University Medical CenterUniversity of KielKielGermany
  2. 2.Institute of Biomedical OpticsUniversity of LübeckLübeckGermany
  3. 3.Department of OphthalmologyUniversity of LübeckLübeckGermany

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