Abstract
Cell-type-specific transcription factors are key to deducing the distinct functions of specialized cells from gene expression profiles. Mesophyll is a major tissue for photosynthesis, and contributes about 80% of total RNA from leaves. Palisade and spongy mesophyll cells are sub-tissues that have different morphologies and physiologies. Thus, determining the palisade and spongy mesophyll-specific transcription factors from the respective sub-tissue-specific transcriptomes is vital to understanding or verifying functions of major plant tissues. One way in which gene expression profiles can be addressed is through direct isolation. Here, we present rapid and simple methods to isolate palisade and spongy mesophyll cells mechanically and enzymatically. This method provides a good yield of each isolated cell type, and the isolated cells can be used for various downstream applications.
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Acknowledgment
We thank T. Koto for technical assistance and J.A. Hejna for English proofreading. This work was supported by JST PRESTO grant 888067 (to M.E.), JSPS KAKENHI grant 16H01240 (to M.E.), grants from the Yamada Science Foundation, Senri Life Science Foundation, and The Nakajima Foundation (to M.E.), and Grants-in-Aid for Scientific Research on Priority Area 25113005 (to T.A.).
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Uemoto, K., Araki, T., Endo, M. (2018). Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells. In: Yamaguchi, N. (eds) Plant Transcription Factors. Methods in Molecular Biology, vol 1830. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8657-6_9
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DOI: https://doi.org/10.1007/978-1-4939-8657-6_9
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