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Plastids pp 205-212 | Cite as

Isolating the Plasmodium falciparum Apicoplast Using Magnetic Beads

  • Cyrille Y. Botté
  • Geoffrey I. McFadden
  • Yoshiki Yamaryo-Botté
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1829)

Abstract

Plastids are key organelles in both photosynthetic and nonphotosynthetic organisms. In photosynthetic organisms, plastids can be readily purified using differential centrifugations due to the high density of photosynthetic membranes or thylakoids. The apicomplexan plastid (the apicoplast) is an essential nonphotosynthetic plastid that lacks thylakoid and was not readily purified using conventional methods. Here, we describe a tractable method to purify intact apicoplasts from Plasmodium falciparum blood stages using magnetic beads and affinity purification.

Key words

Apicomplexa Plasmodium falciparum Apicoplast Purification Magnetic column Magnetic separation 

Notes

Acknowledgments

CYB is a CNRS Research fellow and is supported by Agence Nationale pour la Recherche (ANR-12-PDOC-0028) and ATIP-Avenir-FINOVI (CNRS, INSERM, FINOVI, Project ApicoLipid). YYB is a University of Grenoble-FINOVI Research Fellow (ApicoLipid project). CYB and YYB are supported by Agence Nationale de la Recherche (LABEX PARAFRAP ANR-11-LABX-0024). The authors would like to thank the Etablissement du Sang Français Grenoble for providing red blood cells.

References

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    Botté CY, Yamaryo-Botté Y, Rupasinghe TW et al (2013) Atypical lipid composition in the purified relict plastid (apicoplast) of malaria parasites. Proc Natl Acad Sci U S A 110(18):7506–7511. https://doi.org/10.1073/pnas.1301251110CrossRefPubMedPubMedCentralGoogle Scholar
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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Cyrille Y. Botté
    • 1
  • Geoffrey I. McFadden
    • 2
  • Yoshiki Yamaryo-Botté
    • 1
  1. 1.ApicoLipid Team, Institute for Advanced BiosciencesCNRS UMR5309, Université Grenoble Alpes, INSERM U1209GrenobleFrance
  2. 2.School of BiosciencesUniversity of MelbourneMelbourneAustralia

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