Isolating the Plasmodium falciparum Apicoplast Using Magnetic Beads
Plastids are key organelles in both photosynthetic and nonphotosynthetic organisms. In photosynthetic organisms, plastids can be readily purified using differential centrifugations due to the high density of photosynthetic membranes or thylakoids. The apicomplexan plastid (the apicoplast) is an essential nonphotosynthetic plastid that lacks thylakoid and was not readily purified using conventional methods. Here, we describe a tractable method to purify intact apicoplasts from Plasmodium falciparum blood stages using magnetic beads and affinity purification.
Key wordsApicomplexa Plasmodium falciparum Apicoplast Purification Magnetic column Magnetic separation
CYB is a CNRS Research fellow and is supported by Agence Nationale pour la Recherche (ANR-12-PDOC-0028) and ATIP-Avenir-FINOVI (CNRS, INSERM, FINOVI, Project ApicoLipid). YYB is a University of Grenoble-FINOVI Research Fellow (ApicoLipid project). CYB and YYB are supported by Agence Nationale de la Recherche (LABEX PARAFRAP ANR-11-LABX-0024). The authors would like to thank the Etablissement du Sang Français Grenoble for providing red blood cells.