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Selection of Antibody Fragments Against Structured DNA by Phage Display

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1827)

Abstract

In addition to the canonical B-form structure, DNA can adopt alternative conformations including Z DNA, triplex DNA, as well as G4 and i-Motif quadruplex structures. Such structures have been shown to form in cells in a dynamic manner. Monoclonal antibodies against such structures represent key tools to study the biological functions of these structures. Here we provide protocols for the generation of antibody fragments against structured DNA using phage display selections.

Key words

Structured DNA Secondary structure G-quadruplex i-Motif Antibody fragment Phage display 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Garvan Institute of Medical ResearchSydneyAustralia
  2. 2.Faculty of MedicineSt. Vincent’s Clinical School, University of New South WalesSydneyAustralia

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