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Exploring Dynamics and Noise in Gonadotropin-Releasing Hormone (GnRH) Signaling

  • Margaritis Voliotis
  • Kathryn L. Garner
  • Hussah Alobaid
  • Krasimira Tsaneva-Atanasova
  • Craig A. McArdleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1819)

Abstract

Gonadotropin-releasing hormone (GnRH) acts via G-protein coupled receptors on pituitary gonadotropes. These are Gq-coupled receptors that mediate acute effects of GnRH on the exocytotic secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), as well as the chronic regulation of their synthesis. FSH and LH control steroidogenesis and gametogenesis in the gonads so GnRH mediates control of reproduction by the central nervous system. GnRH is secreted in short pulses and the effects of GnRH on its target cells are dependent on the dynamics of these pulses. Here we provide a brief overview of the signaling network activated by GnRH with emphasis on the use of high content imaging for their examination. We also describe computational approaches that we have used to simulate GnRH signaling in order to explore dynamics, noise, and information transfer in this system.

Key words

GnRH G-protein coupled receptor NFAT ERK Mathematical modeling Mutual information 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Margaritis Voliotis
    • 1
    • 2
  • Kathryn L. Garner
    • 3
  • Hussah Alobaid
    • 3
  • Krasimira Tsaneva-Atanasova
    • 1
    • 2
  • Craig A. McArdle
    • 3
    Email author
  1. 1.EPSRC Centre for Predictive Modeling in HealthcareUniversity of ExeterExeterUK
  2. 2.Department of Mathematics and Living Systems Institute, College of Engineering, Mathematics and Physical SciencesUniversity of ExeterExeterUK
  3. 3.Laboratories for Integrative Neuroscience and Endocrinology, School of Clinical SciencesUniversity of BristolBristolUK

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